I have run some samples on Miseq. After a quality check by FastQC, some of my samples had error with per tile sequence quality at a specific position. If I am not wrong, I think it was caused by the preparation of flowcell becase the samples with this kind of error were shown at the same specific position.
My questions are...
- Please give me some explanations; what is the cause of this error?
- How to solve this problem or how to improve the quality of my sequences?
- Can I ignore this error?
- I have heard that FilterByTile might can help me on this, but I do not know how to use it. Please give me some suggestions
Thank you in advance.