Differential Expression for different GEO datasets
0
0
Entering edit mode
12 weeks ago
Paulyna • 0

Hello,

I'm planning on analyzing different datasets from GEO.

Have to be honest, I've done wet-lab for most of my career, and I'm now slowly moving into "bioinformatics" (quotations because I feel like I'm not doing proper bio info work). I've been coding for almost a year and quite used to R and python now.

My first question is, What is the impact of comparing the expression on different platforms? as not all experiments have been conducted using the same platform

    • I'm not really comparing between different datasets, but want to compare the expression of some genes of interest between different experiments.

3.- Anyone could recommend a good book for statistics in bioinformatics?

I still don't fully understand the whole workflow

Many thanks!

  • A fellow trying to learn bioinformatics whilst doing a PhD
RNA-seq differential_expxression • 181 views
ADD COMMENT
1
Entering edit mode

Modern Statistics for Modern Biology by Susan Holmes and Wolfgang Huber. Otherwise, read the Bioconductor vignettes and workflows.

ADD REPLY
0
Entering edit mode

Can you give a precise example of what you want to compare? Like one gene between groups and each dataset has these groups, or really compare between datasets?

For a book recommendation, you probably want https://www.huber.embl.de/msmb/

ADD REPLY

Login before adding your answer.

Traffic: 2302 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6