Hi Jacques and AGAT users,
I am wondering whether there is a way to maintain identical isoforms when we convert? When I use it removes 28 isoforms (identical). It interferes with my Kallisto/IsoformSwitchAnalyzeR pipeline given that it requires 28 identical isoforms removed during the conversion. Thank you in advance.
PS: I asked the same question there at Github. Apologies for crossposting.
Why are there identical isoforms? What is their biolofical significance?
The original gff3 and fasta files are coming from the potato genome database. I was hesitant to remove those 28 isoforms given they are there in the database. I manually aligned a couple of them just to compare as well. There are no differences and validate the AGAT removal. I guess I need to rerun Kalllisto again to make the Kallisto index without those 28.