I'm performing data analysis of samples of which the RNA has been sequenced. I've gotten pretty significant differences in total gene counts of samples. I've added a picture of a barplot below of the total gene counts of all my samples. The y-scale displays total gene counts divided by 10^6. The two samples with the lowest gene counts have a count of 0.2x10^6, while the sample with the highest gene counts has >20x10^6 counts. Does someone know whether this difference is normal and whether it is advisable that I remove certain outlier samples?
Thanks in advance!