NGS data analysis
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5 weeks ago
bookorg ▴ 10

hi, i am a molecular biologist. I worked on a project on application on tNGS for detection of drug-resistant tuberculosis from clinical samples . So we have target 18 drug activity sites. In that 18 targeted sites the mutation can be occured. For that reason the drugs become resistant. So first we have to sequence that 18 region .After sequencing we have to analyse the mutational pattern.So how can the i perform the analysis. Can anyone help me kindly on that regards?? And i think i also need to know,will i can perform virtual box for the analysis??Please kindly share me some vedios and link

tNGS • 432 views
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5 weeks ago

Puh, that's like asking a engineer how to design and construct a car. I'm afraid to say, that more information are necessary and a more specific question.

Virtual Box is just a software to emulate other machines. By that you can use Windows or Linux and so on, but I do not understand how this is related to your problem.

What exactly is your analysis?

Right know, I could only recommend basic bioinformatics books/lectures.

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Thank you very much for your reply. But I need to know how to do NGS data analysis through linux. I want to learn it. I donot know very much about that. Thats why i am asking for help

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Hire, contract or collaborate with a bioinformatician. You'll spend a ton more time learning how to do basic stuff and a lot less time doing the actual analysis. Focus on designing the experiment and let a pro take care of running the analysis. There are many companies that will work with wet bench biologists for these analyses - search the forum for "Cloud Genomics companies"

What you're looking for is not a simple task that someone on the Internet can help you with.

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