Inferring GRN without transcription factor motif

Question

[William]

Hi everyone,

I'm running pySCENIC on several 10X single cell RNAseq datasets. I know that several TFs have large regulatory roles in the cell types I'm interested in. The TFs come up as having top importance scores during the GRNboost2 step of SCENIC, but dont show up after motif enrichment (CTX). This seems to be because the transcription factors do not have well described binding motifs(don't have motifs in the cistarget DBs or ENCODE. Without doing ChIP-seq and determining motif, what are the best ways to go forward if I would like to get some useful info about the GRNs?

Thanks!