Multiplex PCR Primers
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22 months ago

I want to evaluate individual primers in the multiple primer pair set to determine their compatibility to use as multiplex PCR primers. I found that MFold 3.6 software can find compatible primer pairs among my designed primers. Unfortunately, MFold is a Unix software! what is the solution for me to check these primer pairs online or on windows?
Multiplex_PCR primer_compatibility • 607 views
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You mean this? http://www.unafold.org/mfold/applications/dna-folding-form.php

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I guess the online server. But how to use that for a set of primer pairs (4 pairs) ?

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