Failed quality score and sequence content small RNA-seq data

Question

[Rey]

Hi all,

In FASTA file format, I received clean reads of small RNA-seq data, Hiseq 2000.

I convert them to Fastq via the seqtk to check their quality. The FastQC shows me the below results for all libraries. Is it possible to continue downstream analysis? Do these results are OK? Thank you!

Answer 1

[ATpoint]

It is fine. If you converted from fasta then the quality values are made up and have no meaning. Continue with your analysis as planned.

[Rey]

Thank you!