Gene expression assay Yeast sample prep

Question

[dshdixit]

Hi,

I have been extracting sample for gene expression assay from yeast, but have a question that how do I ensure samples I am collecting at 48 hr. culture time, by then culture is probably saturated. how do i ensure that sample used for extraction will have live(actively dividing cells only)?Is there a procedure to segregate it in yeast?

Thank you in advance

Best,
Disha

[GenoMax]

This question is off-topic for this forum. Please ask in experimental biology reddit or biology stackexchange.

[dshdixit]

Thank you so much!