Dear forumers, I've been trying to generate an ancestral sequence using the ancestral sequence reconstruction software FAstML. I am using the command line and have made sure the input files (both the aligned sequences and the phylogenetic tree) are in correct format, but every time I start the program it terminates, showing the following message:
sh: 1: cannot create outdir/fastml.std: Directory nonexistent
FASTML EXIT ON ERROR: FASTML failed to run, one of the files seq.marginal.txt or prob.marginal.txt were not created...
I've tried different methods, and made sure the files and software were in the right directory but I still keep getting this message.
Any help or insight is appreciated ! Thank you so much.