Hi, I used Blast and Trinotate to annote de novo assembly I constructed using Trinity.
Now, I want to study that transcripts without blast hit (they doesn't appear in the Trinotate output). My question is: How can I exctract that unmapped transcripts? Is there a way to tell blast to output the unmapped reads into a separate file?
I want do it because I'm studdying the presence of new Antimicrobial Peptide Genes in Blatella germanica (it doesn't have a reference genome).
Any advice or suggestion is welcome. Thanks in advance :)