Bimodal read lenghts in pacbio transcriptome sequencing

Question

[Carlos]

Hi guys,

I am analyzing the data sequencing of a RNA-Seq using Pacbio. It was done with two cells (one with < 5kb, and the other 5-10kb) of sequencing. When analyzing the transcript lengths, it produces a bimodal distribution with hardly an overlap between the two modes and one peak at 1 kb and the other at 5.5 kb. Has anybody had the same pattern when analyzing pacbio transcriptome sequencing data?

Best regards,

Carlos