This tiny tutorial cover setting up Aspera Connect (binary is called
ascp) which might be used to download sequencing data, e.g. with download links provided by sra-explorer.info, see also sra-explorer : find SRA and FastQ download URLs in a couple of clicks
Setting up Aspera Connect is simple and was covered in my now deprecated tutorial. However, Aspera Connect versions > 4.2 no longer come with a required private key (
asperaweb_id_dsa.openssh) so I want to share here some links to download versions < 4.2 and some install instructions:
Get the latest version that still works with the download links from sra-explorer.info
#/ Linux wget https://ak-delivery04-mul.dhe.ibm.com/sar/CMA/OSA/0adrj/0/ibm-aspera-connect_126.96.36.199_linux.tar.gz tar zxvf ibm-aspera-connect_188.8.131.52_linux.tar.gz bash ibm-aspera-connect_184.108.40.206_linux.sh #/ macOS -- download and then run the installer (have no Mac to test right now) wget https://ak-delivery04-mul.dhe.ibm.com/sar/CMA/OSA/0adri/0/ibm-aspera-connect_220.127.116.11_macOS_x86_64.dmg
For Linux that creates the binary and openssh key in these locations:
For Mac, after running the installer it should be:
$HOME/Applications/Aspera\ Connect.app/Contents/Resources/ascp $HOME/Applications/Aspera\ Connect.app/Contents/Resources/asperaweb_id_dsa.openssh
Under normal circumstances that should be all it takes. You can now run the download links from sra-explorer, e.g.
ascp -QT -l 300m -P33001 -i $HOME/.aspera/connect/etc/asperaweb_id_dsa.openssh firstname.lastname@example.org:vol1/fastq/SRR144/004/SRR1448774/SRR1448774.fastq.gz .
Let me know if this needs and changes.