I followed the VCF4.2 specification, and created a file in the following structure:
##fileformat=VCFv4.2 #CHROM POS ID REF ALT QUAL FILTER INFO 1 116244010 . T C 137.0 . CO=68 1 116311066 . G A 172.0 . CO=83 1 156100548 . G A 115.0 . CO=51
The file is uploaded to a platform where it gets annotated. I don't know what program or script are used for the annotations, because I can't access them.
The error message I get is the following:
"Annotation failed with the following message: Incorrect header - Some of the VCF header columns are missing".
Can anyone see what may cause the problem with the above structure?
I edited the file according to the comment, yet the error still appears. Now the file looks as follows:
##fileformat=VCFv4.2 ##INFO=<ID=CO,Number=1,Type=Integer,Description="Coverage"> #CHROM POS ID REF ALT QUAL FILTER INFO 1 116244010 . T C 137.0 . CO=68 1 116311066 . G A 172.0 . CO=83 1 156100548 . G A 115.0 . CO=51