I've trimmed rnaseq data (PE 100bp reads) thanks to trimgalore ro remove adapters.
After trimmming I've noticed an increased on GC content (see below), specifically for reverse reads, and for high value of GC %.
I'm having trouble to understand where it came from.
And I wonder if I should worry about it, or maybe avoid trimming step, as adapter contamination is rather low.
Any input is welcome !
before trimming :
after trimming :