RNA-seq: How to get an information about breakpoint position of junction reads
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21 months ago
m166108 • 0

Hi I have a RNA-seq data BAM file. I want to check the Defective viral genome, and I can check the read junction in IGV. But I need a file that record the Reads' breakpoint.

EX: Readname | breakpoint position(5') | breakpoint position(3')

I used to the bedtool bamtobed but it just record the reads position in column 2 & 3

Junction RNA-seq Breakpoint BAM • 303 views
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