Hey Biostars Community,
What are some pros and cons of DNA Mass Spec versus Whole Genome/Exome Sequencing?
The very obvious con is that DNA Mass Spec will only detect the mutations that have been selected to be profiled. Whereas for WGS/WES will be able to profile the whole genome/exome for any mutations including the ones that DNA Mass Spec will be looking at.
Is DNA Mass Spec... I guess... more specific and sensitive (than WES/WGS) for detecting the mutations that it does profile? Thereby giving more confidence in the results from it?
Is there sometimes uncertainty/less confidence with your results from WGS/WES?
I guess an experiment could be done with WGS/WES and then maybe verified by some type of Sanger Sequencing? Thoughts?
Thank you in advance! Pratik