How to perform 6 Frame Translation with Diamond

Question

[m.radz]

For a project I am working on I was given the following advice:

"Six frame translate your fastq reads using diamond and take the top hit against the appropriate ncbi clusters or filter your six frame translated through to a localisation program like psort."

I have used Diamond before as a sequence aligner but I have never used it to perform "6 frame translation". Looking at the documentation I can't work it out either, does anyone have any advice on how to do this?

Answer 1

[GenoMax]

From Diamond manual:

# running a search in blastx mode
./diamond blastx -d reference -q reads.fasta -o matches.tsv 

Align translated DNA query sequences against a protein reference database.