polyA+ versus polyA- RNA seq from ENCODE
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23 months ago
mdwain.uw • 0

I'm trying to download RNA-seq data from the ENCODE portal:

https://www.encodeproject.org/experiments/ENCSR000CPG/

But am struggling to understand what the term "polyA plus RNAseq" and "polyA negative RNAseq" means. I would guess "polyA plus RNAseq" is RNA sequencing of mRNAs with a polyA tail. What I'm confused about is "polyA negative RNAseq". Does this refer to (1) sequencing RNA that lacks a poly A tail, or (2) RiboMinus RNA seq protocols where you subtract out the ribosomal RNAs?

ENCODE rna-seq • 872 views
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Entering edit mode
23 months ago
yhoogstrate ▴ 150

Please read attachment "LID8660.pdf" curefully.

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