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15 days ago

luffy
▴
20

Hello experts,

I have small data where i need calculate statistical significance but since the sample size is very small i am not sure **which method to use** and can i call any of significance because of the small numbers?

Here for example `Gene group 1`

has 14 presented with `phenotype 1`

how can i say this is statistical significant?

Any help would be much appreciated

Thank you for your time

If I understood it correctly, the test you are looking for is Fisher's exact test, in which you compare two groups (Gene group 1 and Gene group 2) and compare among those groups how many have phenotype I and how many don't. You should get a 2X2 table and run the test.

@asaf Thank you for your response. Is it possible to use the other groups as well? the "other gene group" and "All VUS" to compare among all those 4 groups? 4x4 table using the Fisher's exact test? Thank you

You could do that with Chi-square test. Just pay attention to what the test actually tests and how to determine which gene group is actually enriched.