MUSCLE alignment windows error

Question

[sara]

I performed muscle alignment for just 2 dna sequences using mega 11 on windows 10, but I got this error "mega application error finalizing muscle alignment: unable to open file"

Any help?

[Mensur Dlakic]

I think the best way to troubleshoot this is by first doing one of the examples that come with MEGA. If they work, at least you would eliminate any installation problems brought up by @genomax. That would mean that your problem is dataset-specific. For example, the sequences may be too long, or not properly formatted.

[GenoMax]

There is not a lot to go on here but one thing to check is whether your account has write permissions to the working directory. Sounds like muscle may not be able to write the output file.