Hello everyone I am working on single cell data (multiple samples) in which samples are grouped based on the condition (control and treated). For the data analysis, I have been using seurat package for pre-processing, normalization (SCT) and identified multiple clusters. Later, for each cluster, performed "DEGs" analysis based on the condition. From DEGs list, I found that the same gene is differential expressed in multiple cluster. Example : Gstm1 was identified in all three clusters as differential expressed gene during comparison of control and treated. Another gene Acaa1b was found as DEG in 2 clusters.
Cluster 0 Cluster 1 Cluster 2 "Gstm1" 1 1 1 "Acaa1b" 1 1 0
Is it normal in single cell data to identified same gene (significant differential expressed) in multiple clusters?
Please let me know if I am doing something wrong.