I'm interested in quantitative analysis of a gene and it's corresponding protein known to have a crucial role in a bacteria. As much as I understand, we can do quantitative analysis using transcriptomic data (RNA-Seq) and get a list of genes involved in particular disease or organism. I don't understand how we can quantify single gene and it's corresponding protein using bioinformatics approach? As opposed to western blot or immunohistology used for experimental approach. Please help I have to address a reviewer's comment soon.