EDAseq, edgeR libraries cannot be installed in M2 mac
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Entering edit mode
19 months ago

I am having such a weird problem of not being able to install EDAseq or edgeR package from TCGAbiolinks in new M2 mac. Where I did not face any problem with intel based older mac. Is there any solution for this problem right now. I am guessing the packages are not optimized for the ARM-based macs. I am pasting texts from the console.

# install packages
  if (!require("BiocManager", quietly = TRUE))
+   install.packages("BiocManager")

> BiocManager::install("TCGAbiolinks")
'getOption("repos")' replaces Bioconductor standard repositories, see '?repositories' for details

replacement repositories:
    CRAN: https://cran.rstudio.com/

Bioconductor version 3.15 (BiocManager 1.30.18), R 4.2.1 (2022-06-23)
Warning message:
package(s) not installed when version(s) same as current; use `force = TRUE` to re-install:
  'TCGAbiolinks' 
> BiocManager::install("EDASeq")
'getOption("repos")' replaces Bioconductor standard repositories, see '?repositories' for details

replacement repositories:
    CRAN: https://cran.rstudio.com/

Bioconductor version 3.15 (BiocManager 1.30.18), R 4.2.1 (2022-06-23)
Installing package(s) 'EDASeq'
also installing the dependencies ‘GenomicAlignments’, ‘Rhtslib’, ‘rtracklayer’, ‘ShortRead’, ‘Rsamtools’, ‘GenomicFeatures’

Warning: unable to access index for repository https://bioconductor.org/packages/3.15/bioc/bin/macosx/big-sur-arm64/contrib/4.2:
  cannot open URL 'https://bioconductor.org/packages/3.15/bioc/bin/macosx/big-sur-arm64/contrib/4.2/PACKAGES'
Warning: unable to access index for repository https://bioconductor.org/packages/3.15/data/annotation/bin/macosx/big-sur-arm64/contrib/4.2:
  cannot open URL 'https://bioconductor.org/packages/3.15/data/annotation/bin/macosx/big-sur-arm64/contrib/4.2/PACKAGES'
Warning: unable to access index for repository https://bioconductor.org/packages/3.15/data/experiment/bin/macosx/big-sur-arm64/contrib/4.2:
  cannot open URL 'https://bioconductor.org/packages/3.15/data/experiment/bin/macosx/big-sur-arm64/contrib/4.2/PACKAGES'
Warning: unable to access index for repository https://bioconductor.org/packages/3.15/workflows/bin/macosx/big-sur-arm64/contrib/4.2:
  cannot open URL 'https://bioconductor.org/packages/3.15/workflows/bin/macosx/big-sur-arm64/contrib/4.2/PACKAGES'
Warning: unable to access index for repository https://bioconductor.org/packages/3.15/books/bin/macosx/big-sur-arm64/contrib/4.2:
  cannot open URL 'https://bioconductor.org/packages/3.15/books/bin/macosx/big-sur-arm64/contrib/4.2/PACKAGES'
Packages which are only available in source form, and may need compilation of
  C/C++/Fortran: ‘GenomicAlignments’ ‘Rhtslib’ ‘rtracklayer’ ‘ShortRead’ ‘Rsamtools’
Do you want to attempt to install these from sources? (Yes/no/cancel) Yes
installing the source packages ‘GenomicAlignments’, ‘Rhtslib’, ‘rtracklayer’, ‘ShortRead’, ‘Rsamtools’, ‘GenomicFeatures’, ‘EDASeq’

trying URL 'https://bioconductor.org/packages/3.15/bioc/src/contrib/GenomicAlignments_1.32.1.tar.gz'
Content type 'application/x-gzip' length 2220464 bytes (2.1 MB)
==================================================
downloaded 2.1 MB

trying URL 'https://bioconductor.org/packages/3.15/bioc/src/contrib/Rhtslib_1.28.0.tar.gz'
Content type 'application/x-gzip' length 1470492 bytes (1.4 MB)
==================================================
downloaded 1.4 MB

trying URL 'https://bioconductor.org/packages/3.15/bioc/src/contrib/rtracklayer_1.56.1.tar.gz'
Content type 'application/x-gzip' length 4018019 bytes (3.8 MB)
==================================================
downloaded 3.8 MB

trying URL 'https://bioconductor.org/packages/3.15/bioc/src/contrib/ShortRead_1.54.0.tar.gz'
Content type 'application/x-gzip' length 5184736 bytes (4.9 MB)
==================================================
downloaded 4.9 MB

trying URL 'https://bioconductor.org/packages/3.15/bioc/src/contrib/Rsamtools_2.12.0.tar.gz'
Content type 'application/x-gzip' length 2870358 bytes (2.7 MB)
==================================================
downloaded 2.7 MB

trying URL 'https://bioconductor.org/packages/3.15/bioc/src/contrib/GenomicFeatures_1.48.4.tar.gz'
Content type 'application/x-gzip' length 1183732 bytes (1.1 MB)
==================================================
downloaded 1.1 MB

trying URL 'https://bioconductor.org/packages/3.15/bioc/src/contrib/EDASeq_2.30.0.tar.gz'
Content type 'application/x-gzip' length 1036427 bytes (1012 KB)
==================================================
downloaded 1012 KB

* installing *source* package ‘Rhtslib’ ...
** using non-staged installation via StagedInstall field
** libs
cd "htslib-1.7" && make -f "/Library/Frameworks/R.framework/Resources/etc/Makeconf" -f "Makefile.Rhtslib"
Makefile.Rhtslib:131: warning: overriding commands for target `.c.o'
/Library/Frameworks/R.framework/Resources/etc/Makeconf:168: warning: ignoring old commands for target `.c.o'
clang -arch arm64 -falign-functions=64 -Wall -g -O2 -fpic -I. -I/opt/R/arm64/include -D_FILE_OFFSET_BITS=64 -c -o kfunc.o kfunc.c
clang -arch arm64 -falign-functions=64 -Wall -g -O2 -fpic -I. -I/opt/R/arm64/include -D_FILE_OFFSET_BITS=64 -c -o knetfile.o knetfile.c
clang -arch arm64 -falign-functions=64 -Wall -g -O2 -fpic -I. -I/opt/R/arm64/include -D_FILE_OFFSET_BITS=64 -c -o kstring.o kstring.c
clang -arch arm64 -falign-functions=64 -Wall -g -O2 -fpic -I. -I/opt/R/arm64/include -D_FILE_OFFSET_BITS=64 -c -o bcf_sr_sort.o bcf_sr_sort.c
clang -arch arm64 -falign-functions=64 -Wall -g -O2 -fpic -I. -I/opt/R/arm64/include -D_FILE_OFFSET_BITS=64 -c -o bgzf.o bgzf.c
bgzf.c:1026:16: warning: variable 'size' set but not used [-Wunused-but-set-variable]
    int count, size = 0, block_length, remaining;
               ^
1 warning generated.
clang -arch arm64 -falign-functions=64 -Wall -g -O2 -fpic -I. -I/opt/R/arm64/include -D_FILE_OFFSET_BITS=64 -c -o errmod.o errmod.c
clang -arch arm64 -falign-functions=64 -Wall -g -O2 -fpic -I. -I/opt/R/arm64/include -D_FILE_OFFSET_BITS=64 -c -o faidx.o faidx.c
clang -arch arm64 -falign-functions=64 -Wall -g -O2 -fpic -I. -I/opt/R/arm64/include -D_FILE_OFFSET_BITS=64 -c -o hfile.o hfile.c
clang -arch arm64 -falign-functions=64 -Wall -g -O2 -fpic -I. -I/opt/R/arm64/include -D_FILE_OFFSET_BITS=64 -c -o hfile_net.o hfile_net.c
echo '#define HTS_VERSION "1.7"' > version.h
clang -arch arm64 -falign-functions=64 -Wall -g -O2 -fpic -I. -I/opt/R/arm64/include -D_FILE_OFFSET_BITS=64 -c -o hts.o hts.c
clang -arch arm64 -falign-functions=64 -Wall -g -O2 -fpic -I. -I/opt/R/arm64/include -D_FILE_OFFSET_BITS=64 -c -o hts_os.o hts_os.c
clang -arch arm64 -falign-functions=64 -Wall -g -O2 -fpic -I. -I/opt/R/arm64/include -D_FILE_OFFSET_BITS=64 -c -o md5.o md5.c
clang -arch arm64 -falign-functions=64 -Wall -g -O2 -fpic -I. -I/opt/R/arm64/include -D_FILE_OFFSET_BITS=64 -c -o multipart.o multipart.c
clang -arch arm64 -falign-functions=64 -Wall -g -O2 -fpic -I. -I/opt/R/arm64/include -D_FILE_OFFSET_BITS=64 -c -o probaln.o probaln.c
clang -arch arm64 -falign-functions=64 -Wall -g -O2 -fpic -I. -I/opt/R/arm64/include -D_FILE_OFFSET_BITS=64 -c -o realn.o realn.c
clang -arch arm64 -falign-functions=64 -Wall -g -O2 -fpic -I. -I/opt/R/arm64/include -D_FILE_OFFSET_BITS=64 -c -o regidx.o regidx.c
clang -arch arm64 -falign-functions=64 -Wall -g -O2 -fpic -I. -I/opt/R/arm64/include -D_FILE_OFFSET_BITS=64 -c -o sam.o sam.c
clang -arch arm64 -falign-functions=64 -Wall -g -O2 -fpic -I. -I/opt/R/arm64/include -D_FILE_OFFSET_BITS=64 -c -o synced_bcf_reader.o synced_bcf_reader.c
clang -arch arm64 -falign-functions=64 -Wall -g -O2 -fpic -I. -I/opt/R/arm64/include -D_FILE_OFFSET_BITS=64 -c -o vcf_sweep.o vcf_sweep.c
clang -arch arm64 -falign-functions=64 -Wall -g -O2 -fpic -I. -I/opt/R/arm64/include -D_FILE_OFFSET_BITS=64 -c -o tbx.o tbx.c
clang -arch arm64 -falign-functions=64 -Wall -g -O2 -fpic -I. -I/opt/R/arm64/include -D_FILE_OFFSET_BITS=64 -c -o textutils.o textutils.c
clang -arch arm64 -falign-functions=64 -Wall -g -O2 -fpic -I. -I/opt/R/arm64/include -D_FILE_OFFSET_BITS=64 -c -o thread_pool.o thread_pool.c
clang -arch arm64 -falign-functions=64 -Wall -g -O2 -fpic -I. -I/opt/R/arm64/include -D_FILE_OFFSET_BITS=64 -c -o vcf.o vcf.c
vcf.c:641:14: warning: variable 'needs_sync' set but not used [-Wunused-but-set-variable]
    int len, needs_sync = 0, done = 0;
             ^
vcf.c:2826:50: warning: variable 'ret' set but not used [-Wunused-but-set-variable]
    int i, ndst_ori = dst->nhrec, need_sync = 0, ret = 0;
                                                 ^
2 warnings generated.
clang -arch arm64 -falign-functions=64 -Wall -g -O2 -fpic -I. -I/opt/R/arm64/include -D_FILE_OFFSET_BITS=64 -c -o vcfutils.o vcfutils.c
clang -arch arm64 -falign-functions=64 -Wall -g -O2 -fpic -I. -I/opt/R/arm64/include -D_FILE_OFFSET_BITS=64 -c -o cram/cram_codecs.o cram/cram_codecs.c
cram/cram_codecs.c:1268:19: warning: variable 'ntot' set but not used [-Wunused-but-set-variable]
    int nvals, i, ntot = 0, max_val = 0, min_val = INT_MAX, k;
                  ^
1 warning generated.
clang -arch arm64 -falign-functions=64 -Wall -g -O2 -fpic -I. -I/opt/R/arm64/include -D_FILE_OFFSET_BITS=64 -c -o cram/cram_decode.o cram/cram_decode.c
clang -arch arm64 -falign-functions=64 -Wall -g -O2 -fpic -I. -I/opt/R/arm64/include -D_FILE_OFFSET_BITS=64 -c -o cram/cram_encode.o cram/cram_encode.c
clang -arch arm64 -falign-functions=64 -Wall -g -O2 -fpic -I. -I/opt/R/arm64/include -D_FILE_OFFSET_BITS=64 -c -o cram/cram_external.o cram/cram_external.c
clang -arch arm64 -falign-functions=64 -Wall -g -O2 -fpic -I. -I/opt/R/arm64/include -D_FILE_OFFSET_BITS=64 -c -o cram/cram_index.o cram/cram_index.c
clang -arch arm64 -falign-functions=64 -Wall -g -O2 -fpic -I. -I/opt/R/arm64/include -D_FILE_OFFSET_BITS=64 -c -o cram/cram_io.o cram/cram_io.c
cram/cram_io.c:61:10: fatal error: 'lzma.h' file not found
#include <lzma.h>
         ^~~~~~~~
1 error generated.
make[1]: *** [cram/cram_io.o] Error 1
make: *** [htslib] Error 2
ERROR: compilation failed for package ‘Rhtslib’
* removing ‘/Library/Frameworks/R.framework/Versions/4.2-arm64/Resources/library/Rhtslib’
ERROR: dependency ‘Rhtslib’ is not available for package ‘Rsamtools’
* removing ‘/Library/Frameworks/R.framework/Versions/4.2-arm64/Resources/library/Rsamtools’
ERROR: dependency ‘Rsamtools’ is not available for package ‘GenomicAlignments’
* removing ‘/Library/Frameworks/R.framework/Versions/4.2-arm64/Resources/library/GenomicAlignments’
ERROR: dependencies ‘Rsamtools’, ‘GenomicAlignments’ are not available for package ‘rtracklayer’
* removing ‘/Library/Frameworks/R.framework/Versions/4.2-arm64/Resources/library/rtracklayer’
ERROR: dependencies ‘Rsamtools’, ‘GenomicAlignments’, ‘Rhtslib’ are not available for package ‘ShortRead’
* removing ‘/Library/Frameworks/R.framework/Versions/4.2-arm64/Resources/library/ShortRead’
ERROR: dependency ‘rtracklayer’ is not available for package ‘GenomicFeatures’
* removing ‘/Library/Frameworks/R.framework/Versions/4.2-arm64/Resources/library/GenomicFeatures’
ERROR: dependencies ‘ShortRead’, ‘Rsamtools’, ‘GenomicFeatures’ are not available for package ‘EDASeq’
* removing ‘/Library/Frameworks/R.framework/Versions/4.2-arm64/Resources/library/EDASeq’

The downloaded source packages are in
    ‘/private/var/folders/3g/tfj46mf17v15t515hjnb1c3m0000gn/T/RtmplSzhiv/downloaded_packages’
Warning messages:
1: In install.packages(...) :
  installation of package ‘Rhtslib’ had non-zero exit status
2: In install.packages(...) :
  installation of package ‘Rsamtools’ had non-zero exit status
3: In install.packages(...) :
  installation of package ‘GenomicAlignments’ had non-zero exit status
4: In install.packages(...) :
  installation of package ‘rtracklayer’ had non-zero exit status
5: In install.packages(...) :
  installation of package ‘ShortRead’ had non-zero exit status
6: In install.packages(...) :
  installation of package ‘GenomicFeatures’ had non-zero exit status
7: In install.packages(...) :
  installation of package ‘EDASeq’ had non-zero exit status
> library(EDAseq)
Error in library(EDAseq) : there is no package called ‘EDAseq’
TCGAbiolinks BiocManager • 1.4k views
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Can you install samtools via conda? That should include the lzma.h headers.

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19 months ago
Gordon Smyth ★ 7.0k

See https://stat.ethz.ch/pipermail/bioc-devel/2022-September/019178.html

PS. I'm curious why you don't sent such a Bioconductor-specific question as this to the Bioconductor support site at https://support.bioconductor.org ? This question has been asked many times on that forum, see for example the response from one of the Bioconductor core team: https://support.bioconductor.org/p/9137290/#9137342
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The problem is resolved by installing the intel version R. Thanks!

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Yes, that's right. But the point of the bioc-devel post that I linked to is that, starting from today, you shouldn't need to install the intel version in order to resolve the issue. From today, Bioconductor installation should work for arm64 R as well.

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