Hello, I was trying to obtain scRNA-seq raw data from SRR11181957: https://trace.ncbi.nlm.nih.gov/Traces/?view=run_browser&acc=SRR11181957&display=data-access
The fastq file should be R1 of 101.2G and R2 of 271.2G.
I tried to get the fastq files directly from ebi, but there is only one fastq.gz file of ~400G, so I'm not sure what it is and how to utilize it.
I then tried SRA tools.
Firstly I used
wget https://sra-pub-run-odp.s3.amazonaws.com/sra/SRR11181957/SRR11181957 to and get a file ‘SRR11181957’ of size 93.0G (however, the ncbi website indicates it should be 86.6G)
Then I added .sra to the file and did (originally tried to get it directly via fasterq-dump SRR11181957 but got errors so I resorted to doing it locally):
export PATH=/sratoolkit.3.0.0-ubuntu64/bin fasterq-dump --split-3 /SRR11181957.sra
Now I have a file SRR11181957_1.fastq of 173.9G and a SRR11181957_2.fastq of 358.8G.
I'm not sure why my files have much larger size and whether they are of the correct size?