Why do I see sudden rise in TOTAL COUNT of my bam. I dont think it is duplication?
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2.2 years ago
pragnapcu ▴ 10

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As you can see the below bam, there is increase in total counts at all exons of a particular gene. I dont think it is duplication. The above sample is from the same sequencing run. As the first sample is normal, I dont think it is sequencing run problem too. So, what might be the possible reason for this elevation? Please share ur thoughts

BAM IGV GATK duplication • 850 views
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What kind of assay is that and what is the difference between the upper and lower panel? In the lower one it is clearly elevated at exactly the exon boundaries. What are the data?

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It is exome data, showed in IGV (Integrative Genomics Viewer). Upper and lower panels are two different samples data. Yes. In the lower one, the read count is elevated exactly at boundaries. I am trying to find out the cause of this.

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Wouldn't the reads between exons just be background, so your second panel has a better exon reads to noise ratio?

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I am not sure if we can interpret so. I am still puzzled and looking for an answer

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