Hi All,
I've used MAKER to produce some gene predictions on some of my largest contigs. I then subsequently saved the FASTA files it produced and used them to run a BLAST, with my query protein sequences being CYP genes found on NCBI. I am trying to analyse the results from this to determine whether I have any matches that are significant and indicate that I have some CYP genes in these contigs but I'm unsure how to determine, using the e-value, whether they are matches. Can anyone give me some idea how to analyse the BLAST results to determine this.
Thanks!