I have single cell RNA seq data from 2 samples. One is control and the other one is treated. I am trying to analyze the data with Seurat. I did the QC analysis, normalized each sample and then I did the integration. I got the clusters and assigned cell types. Now I would like to do the DE analysis between the control and treated CD4 cells. Can I do DE analysis if I only have one sample per condition? Does it make sense statistically to do it? I am planning to do it anyway as exploratory data analysis but I was wondering how much can I trust the p values that come out of that test.