GC content RNA-seq
0
0
Entering edit mode
12 weeks ago
UserA • 0

Hello, For one project, we sent a large number of samples, each containing 2 or 3 bacterial strains, to the illumina NovaSeq 6000 paired-end sequencer. For some samples, I observe on the GCcontent graph as 2 peaks close together. Could this be due to the fact that my strains do not have the same GC%? In this case why don't I observe it on the other samples?

enter image description here

RNAseq GC • 207 views
ADD COMMENT
2
Entering edit mode

that my strains do not have the same GC%?

That is one possibility.

In this case why don't I observe it on the other samples?

It is possible that you have varying amounts of rRNA and that may be resulting in the doublets.

ADD REPLY

Login before adding your answer.

Traffic: 1439 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6