t2t human reference genome for RNA-seq
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19 months ago
bompipi95 ▴ 150

Hi bioinformaticians!

With the complete telomere-to-telomere assembly of the human genome now available, has anyone tried aligning their RNA-seq reads to the t2t human reference (using STAR for instance), and comparing against the hg38 results to see if they get more interesting differentially expressed genes/repeats? Curious about your thoughts and experience on the utility of this assembly for transcriptomic analysis.

t2t hs1 RNA-seq • 782 views
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