Sylamer is a method for detecting microRNA target and small interfering RNA off-target signals in 3' untranslated regions from a ranked gene list, sorted from upregulated to downregulated, after a microRNA perturbation or RNA interference experiment. want to use Sylamer for identifying the effect of microRNA effect on the genes that popped up as differential expressed from an RNA-seq data.As I have no experience of using this type of software I would like some guidance or help regarding using this tool, (https://pubmed.ncbi.nlm.nih.gov/18978784/) this paper explains the use of this software. (https://www.genomique.info/sylamer/) is also a site that need some data input and give you a result but I am not sure if I am using it correctly.Would it be possible to get one guidance related to this analysis?I would be highly grateful if anyone can help me. Kind regards!
