Karlin-Altschul parameter error
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11 days ago
aus • 0

Has anyone had this problem with blastn (newest version)?

Trying 14mil reads to the ref_viruses_rep_genomes database. Has worked perfectly in the past but not with a new blast install and newest db.

If I do smaller number of reads or if I put the word_size at less than 11 I don't get this error:

Could not calculate ungapped Karlin-Altschul parameters due to an invalid query sequence or its translation. Please verify the query sequence(s) and/or filtering options

Used to work fine - in less than 5 mins to run all 14mil reads. Help!

Karlin-Altschul • 173 views
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due to an invalid query sequence

Have you checked on this? Perhaps you have a corrupt query file. What is the significance of 14M?

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