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17 months ago
aus
•
0
Has anyone had this problem with blastn (newest version)?
Trying 14mil reads to the ref_viruses_rep_genomes database. Has worked perfectly in the past but not with a new blast install and newest db.
If I do smaller number of reads or if I put the word_size at less than 11 I don't get this error:
Could not calculate ungapped Karlin-Altschul parameters due to an invalid query sequence or its translation. Please verify the query sequence(s) and/or filtering options
Used to work fine - in less than 5 mins to run all 14mil reads. Help!
Have you checked on this? Perhaps you have a corrupt query file. What is the significance of 14M?