Creating a phylogenetic tree following MSA

Question

[borenstin.tom]

Hi, Using my windows machine I was able to use Clustalo command line tool to conduct MSA on 1,000 different protein sequences (around 30 - 40 A.A long). At this point I wish to use the aligned output to produce a phylogeneitc tree. I would love to hear recomendations on which tool would be the best fit for this job? I plan on using more sequences in the future so a tool which can handle bigger scales of work has an advantage.

Answer 1

[Mensur Dlakic]

For that many sequences, and especially considering they are short, an educated guess is that many of them are identical. If so, there is no point in including them all in a tree. You will end up with branches that have several leaves of zero length, because there is no distance between the sequences. You can get the same information from the fact that they are identical.

Besides, removing identical sequences will also make a tree more amenable to visual inspection. I have looked at the trees all the way from a couple of entries to tens of thousands. To my eyes, trees that have more than 150-200 entries are very difficult to inspect, and those over a thousand are almost impossible to grasp in a meaningful way. Cutting out the redundant sequences should help with that.

https://sites.google.com/view/cd-hit

There is a list of all phylogeny-related programs:

https://evolution.genetics.washington.edu/phylip/software.html

Most of them will not have any problem with the dataset size you have. I recommend IQ-TREE from the Maximum Likelihood list and MrBayes among Bayesian inference programs. MEGA will probably do fine if your main feature of interest is user-friendliness.

Answer 2

[Xylanaser]

Try this: https://www.megasoftware.net/downloads/dload_win_gui

[Xylanaser]

i recommendusing linux and cmd :)