modified bases in oligo/primer?
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Entering edit mode
8 weeks ago
yb8d • 0

Hi All,

Can someone remind me what the Asterix (*), such as those appearing at the end of the line, mean in an oligo such as:

CAAGCAGAAGACGGCATACGAGATNNNNNNNNGTGACTGGAGTTCAGAC GTGTGCTCTTCCGATCTTCTACTATTCTTTCCCCTGCA*C*T*G*T


Best regards,

Yongde

chemistry DNA • 171 views
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Entering edit mode
8 weeks ago
ATpoint 68k

It usually stands for modified bases. I know them as PTO bonds when you order primers/oligos, but probably it is any modified base and you need to check the legend of the sequence provider.