Using Mutsigcv When Mutation Data Is Not Sufficient

Question

[liyfpaper]

Dear all:

I have a problem when using MutsigCV.

I sequenced 20 paired tumor and normal tissues, and called somatic mutations including missense, nonsense, splicing and synoymous. And this is sufficient for MutsigCV, as I have synoymous mutation data.

Next, I wish to use the data from published paper, however, I found that there are no synoymous mutations there. Only missense, nonsense, splicing mutations are included. How can I integrate my data and published data when using MutsigCV? Thank you!

Li

[Giovanni M Dall'Olio]

To which paper do you refer?

[liyfpaper]

Thank you for your attention, Giovanni. The paper is Exome sequencing of liver fluke–associated cholangiocarcinoma. The paper do not contain synonymous mutations.