Entering edit mode
2.3 years ago
joonhong kwon
▴
70
Hi all,
I have a question about the relationship between DEG and cell number (or proportion or frequency) comparison in scRNA-seq. DEG analysis and comparison of cell number were performed to compare control and disease group. When pseudo-bulk DEG analysis was performed using DESeq2, gene A was up-regulated in Cluster X. But cell number of Cluster X was decreased in disease group.
In this case, can it be said that gene A was actually up-regulated in the disease group? Or what if we want to show that gene A expression is increased?
Thank you.
Best,
Joonhong
