When I run Trimmomatic, I continue to get an "unknown trimmer" error and cannot figure out how to fix it.

Question

[littlemicrobiologist]

I have applied following in the folder where the data is:

java -jar Trimmomatic-0.39/trimmomatic-0.39.jar PE phred33 /home/*/*R1.fastq.gz /home/*/*R2.fastq.gz *R1.trimmed.fastq.gz *R1.untrimmed.fastq.gz *R2.trimmed.fastq.gz *R2.untrimmed.fastq.gz ILLUMINACLIP:/home/Trimmomatic/adapters/TruSeq3-SE.fa:2:30:7 MINLEN:15

the output i got:

TrimmomaticPE: Started with arguments:
phred64 /home/*/*R1.fastq.gz /home/*/*R2.fastq.gz *R1.trimmed.fastq.gz *R1.untrimmed.fastq.gz *R2.trimmed.fastq.gz *R2.untrimmed.fastq.gz ILLUMINACLIP:/home/trimmomatic/adapters/TruSeq3-SE.fa:2:30:7 MINLEN:15
Exception in thread "main" java.lang.RuntimeException: Unknown trimmer: *R2.untrimmed.fastq.gz
    at org.usadellab.trimmomatic.trim.TrimmerFactory.makeTrimmer(TrimmerFactory.java:73)
    at org.usadellab.trimmomatic.Trimmomatic.createTrimmers(Trimmomatic.java:59)
    at org.usadellab.trimmomatic.TrimmomaticPE.run(TrimmomaticPE.java:552)
    at org.usadellab.trimmomatic.Trimmomatic.main(Trimmomatic.java:80)

[Pierre Lindenbaum]

don't use those stars and use the real files without globbing , and then update your post for a start.

[littlemicrobiologist]

Hi, I used real files only. here I just pasted the format that I used while trimming.

[GenoMax]

Even if you used real file names, home/*/ seems like a suspect path. Do you have a directory called home in which ever directory you are running this from? Looks like that may need to start with a /home if you are referring to files in your home directory.

A similar issue may exist in this path trimmomatic/adapters/TruSeq3-SE.fa.

[littlemicrobiologist]

Thanks, but it still showing unknown trimmer: <the output file>

[GenoMax]

Assuming you have the right number of options provided this is going to be a path issue. Make sure the file with adapter sequence exists. Provide full path to /full_path_to/TruSeq3-SE.fa.

[littlemicrobiologist]

hi, I provide full path, but it still shows same

[lieven.sterck]

Can you post the commandline again here, after you have fixed/implemented all of GenoMax remarks.

Thanks

[Ram]

Do not delete posts that have received feedback. Interact with the people that have invested effort into helping you, and work towards providing closure to your post.