How to identify basic attributes of an mRNA
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9 weeks ago
OLAOYE • 0

Hi everyone. I have got an mRNA sequence and I wish to know where is the:

1. Ribosome Binding Site
2. Alternative Start Site
3. Promoter Sequence.
Ribosome • 447 views
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9 weeks ago
Mensur Dlakic ★ 23k

Tough to help you without knowing whether we are talking prokaryotes or eukaryotes here. Here is a guideline to RBS in both:

https://en.wikipedia.org/wiki/Ribosome-binding_site

The idea is to first find a start codon, and RBS is usually not too far upstream. The same is true for alternative start sites, if there are any.

Promoters are not transcribed - they provide signals that drive transcription. There will be no promoters in mRNA sequences.

The title is is completely non-informative. Maybe something like How to find ribosome binding sites? would be more appropriate?