I am trying to use the POSSUM Bioinformatics tool locally as I want to generate PSSM features using the UniRef100 database as my reference db. POSSUM documentation says that I will need to specify the location of my reference db pssm files aka where I have my UniRef100 pssm files but I can't figure out how to generate or find UniRef100 pssm files online anywhere.
I have the POSSUM stand alone toolkit set up and working, and have successfully tested with the example files. Ideally I would like to generate pssm descriptors by running my aligned sequences against the UniRef100 database. Can anyone help point me in the right directions as to how one would generate a pssm of a reference database like UniRef100 or where I can find it to download? The links I have found searching similar posts here that mention downloads or API's are broken.
Okay I will give it a try, thank you so much for your response!