I've seen many posts and questions regarding the error flagged by fastqc on "Per base sequence content". My understanding is the variation at the sequence content at the front end is a normal occurrence for RNAseq on Illumina due to random priming, however, I couldn't find a straight answer if this is also normal for DNA seq?
I have done metagenomic sequencing (DNA) in which the library was made using NEBNext Ultra II FS Kit. And fastqc did flag warning for "Per base sequence content".
I attached the Per base sequence content plot here accessible from the link below.
Is this expected for DNA sequencing?