I am analyzing my single nuclei dataset. I did clustering and cluster annotation. And I observed sth that I couldn't explain why it is happening. Below is the umap projection of my dataset. As you see the MN_1 cluster is (green one) is clustered separately. But some of cells are closer to oligodendrocyte clusters (the blue one) than MN cluster. I don't know first, why the MN_1 is one cluster but splitted into two cluster second, why some of its cell seems to be part of Olig clusters
Any help is really appreciated!