Hi bioinformaticians, would anyone calculate RPKM from the count matrix with edgeR or DESeq2?
I found some resources guide on this but there are one or two steps I don't know such as this:
y <- DGEList(counts=counts,genes=data.frame(Length=GeneLength)) y <- calcNormFactors(y) RPKM <- rpkm(y)
How to get GeneLength?
How to get annot object?
I appreciate your help!