Entering edit mode
10 weeks ago
diqixiaoyaoer ▴ 10
I just practiced the scRNAseq upstream analysis from cellranger official tutorial.
But I met a small problem but it stopped me from going the next step.
I followed the tutorial exactly but the error information still exist:
## cellranger count --id=run_count_1kpbmcs \ --fastqs=/home/user/Desktop/run_cellranger_count/pbmc_1k_v3_fastqs \ --sample=pbmc_1k_v3 \ --transcriptome= /home/user/Desktop/run_cellranger_count/refdata-gex-GRCh38-2020-A error: Found argument '/home/user/Desktop/run_cellranger_count/refdata-gex-GRCh38-2020-A' which wasn't expected, or isn't valid in this context USAGE: cellranger count [OPTIONS] --id <ID> --transcriptome <PATH> For more information try --help
I tried many methods but I didn't know what's wrong with it .
Can somebody help me solve this problem. I'd be appreciate it.
Oh, yes. I got it. It wasted me mucht time. But I think the problem reason is ridiculous.
I am really thankful for you help.