desgin a primer for CDS
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18 months ago
yuxiang • 0

I want to desgin a primer for the mRNA, now I have some cell samples, it contains gDNA, mRNA(not only the target gene) More detail: I need to use the pcr to amplyfy the target gene mRNA(mature), now I need to design a primer which can produce the CDS( mRNA).

gene pcr • 931 views
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what is the question ? are you looking for a software ? good practices ?

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sorry for my late reply, I am a student, my profossor ask me to dsgin a primer for the specific cDNA, I have solved now, thanks for your reply.

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how can you know the primer is not bind to other gene site? how can you desgin an experiment? I use the FISH to identify the gene, how about you?

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