Entering edit mode
11 months ago
safeassli
•
0
Good afternoon,
I'm working with single cell data for the first time so I'm not an expert in this field. I have an Integrated data assay ( Lup.combined <- IntegrateData(anchorset = Lup.anchors)) I determined the different clusters and I annotated them. My question is how I can perform pseudo bulk analysis on this type of data.
I know that pseudo bulk must be performed on non normlized raw counts but how I can go from this integrated data to row counts while keeping the annotation and clustering columns?