Hello, I know rna seq in general has 3 prime bias. When I say rna-seq, i am talking about illumina, oxford nanopore, and pacBio. Here are my questions.
- As I know, illumina begins sequencing from both 5 prime and 3 prime but why are there 3 prime bias?
- I heard ONT has 3 prime bias the most, then illumina, and then pacbio. Is this because pacbio is longer (2k bp) than ONT (660bp) and begins sequencing from 3 prime end?
Can someone explain these two questions?