How the "IJC" is calculated in rMATS tool?
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11 months ago
Apex92 ▴ 280

Dear all, I ran rMATS on my bams files to detect potential intron retention In my genes.

In the rMATs report I have the IJC (number of reads that mapped to the exon inclusion isoform) numbers for each of my samples. Based on the reported introns by rMATS I also extracted the intron coordinates and calculated the number of reads that mapped to the intron but my numbers (number of reads that mapped to a specific intron) are different (sometimes a big difference) with the IJC values of rMATS for a specific intron. I have not found an explanation for this, could someone please help me to know why there is this difference and how the IJC is exactly calculated?

Thanks you.

Sashimi sequencing alignment rMATS rna-seq • 453 views
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