selection of Gene Ontology terms manually
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11 months ago
harish • 0

I am new to RNAseq analysis. I am performing GO analysis using Clusterprofiler in r and get some 100 GO terms, all within the cutoff of 0.05 set (gene list has more than 3K genes). Can I manually select 10 GO terms and plot them from the top 20 GO terms? My aim is to give the audience a snapshot of GO terms interesting to me at the top of the list and also to only include either parent or child GO terms. Would it be considered biased, or is it unethical, if so then why does the Clusterprofiler have a function to manually select the GO terms for plotting using showCategory? should I continue with it or should I just select the top 10 terms.

RNAseq Gene-ontology GOanalysis Clusterprofiler • 372 views
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