I have a Fasta file of the chicken genome sequence which size is 54 GB. I am trying to do the assembly of this chicken genome using Abyss using the following code-
abyss-pe k=32 B=25G name=assembly in='merged_female.fa'
I gave B=25G as the chicken genome has around 1.2 × 10^9 base pairs and k=32 to reduce memory usage. I have 122Gi memory available in the system. I got this error-
abyss-rresolver-short -b25G -f0.8 -j2 -k32 \ -h assembly-1-rr --dot -c assembly-1-rr.fa \ -g assembly-1-rr.dot assembly-1.fa assembly-1.dot merged_female.fa assembly-1.fa:0: warning: file is empty make: *** [/home/mkk/.conda/envs/abyss-env/bin/abyss-pe.Makefile:583: assembly-1-rr.dot] Segmentation fault (core dumped)
I'd be thankful if someone can give me a solution for this problem.