readGenericHeader() error message Limma

Question

[survive]

I am trying to process Agilent data by using limma R package. For GSE8842, I used the following code:

raw_data <- read.maimages(target_file, source = "agilent")

but I got the following error:

Error in readGenericHeader(fullname, columns = columns, sep = sep) :
  Specified column headings not found in file

I tried to rename the colnames of the file to match the format, such as "Green Background" to "G", "Red Signal" to "R"; but I still receive the errors..

wondering how can I solve the errors.

Thank you.

[Raony Guimarães]

Can you post the error you are getting after renaming the colnames?

[survive]

Hi, I got the same error:

Error in readGenericHeader(fullname, columns = columns, sep = sep) : 
  Specified column headings not found in file

I am thinking rather there is a problem with the original raw data.